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Metabolic behavior of immobilized Candida guilliermondii cells during batch xylitol production from sugarcane bagasse acid hydrolyzate
Author(s) -
Carvalho Walter,
Silva Silvio S.,
Converti Attilio,
Vitolo Michele
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10319
Subject(s) - xylitol , bagasse , xylose , chemistry , fermentation , food science , yeast , biomass (ecology) , yield (engineering) , biochemistry , microbiology and biotechnology , biology , agronomy , materials science , metallurgy
Candida guilliermondii cells, immobilized in Ca‐alginate beads, were used for batch xylitol production from concentrated sugarcane bagasse hydrolyzate. Maximum xylitol concentration (20.6 g/L), volumetric productivity (0.43 g/L · h), and yield (0.47 g/g) obtained after 48 h of fermentation were higher than similar immobilized‐cell systems but lower than free‐cell cultivation systems. Substrates, products, and biomass concentrations were used in material balances to study the ways in which the different carbon sources were utilized by the yeast cells under microaerobic conditions. The fraction of xylose consumed to produce xylitol reached a maximum value (0.70) after glucose and oxygen depletion while alternative metabolic routes were favored by sub‐optimal conditions. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 165–169, 2002.