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Production of lipolyzed butteroil by a calf pregastric esterase immobilized in a hollow fiber reactor: III. Effect of glycerol
Author(s) -
Lessard Louis P.,
Hill Charles G.
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10291
Subject(s) - chemistry , glycerol , chromatography , esterase , lipolysis , kinetics , fatty acid , fiber , enzyme , biochemistry , organic chemistry , adipose tissue , physics , quantum mechanics
Lipolysis of butter oil in a hollow fiber reactor containing an immobilized calf pregastric esterase was studied at 40°C, a pH of 6.0, and glycerol concentrations of 0, 150, and 500 g/L in the buffer solution. The concentrations of 10 fatty acid species in the lipolyzed product were determined using high‐performance liquid chromatography. The rate of loss of enzyme activity and the relative selectivities of this esterase depended on the glycerol concentration. By contrast, the overall rate of release of fatty acids was not affected by the glycerol concentration. Loss of enzyme activity was modeled using first‐order kinetics. The models for deactivation and reaction kinetics were fit simultaneously to the data. The model was successful in describing the rates of release of all 10 fatty acid species for a range of space times from 0 to 25 h. The parameters of the model were tested for dependence on glycerol concentration. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 323–333, 2002.