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Scale‐up of pseudo solid‐phase enzymatic synthesis of α‐methyl glucoside acrylate
Author(s) -
Li Yanzi,
Rethwisch David G.
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10272
Subject(s) - candida antarctica , acetone , chemistry , lipase , transesterification , methyl acrylate , chromatography , organic chemistry , acrylate , solvent , triacylglycerol lipase , glucoside , enzyme , methanol , monomer , medicine , alternative medicine , pathology , polymer
The successful scale‐up of the enzymatic synthesis of α‐methyl glucoside acrylate from laboratory‐scale (milliliter) to pilot‐scale (liter) was examined. Specifically, Candida antarctica lipase B (Novozym 435) was used as a biocatalyst to produce α‐methyl glucoside acrylate via the transesterification of α‐methyl glucoside (MG) with vinyl acrylate (VA) using acetone as a solvent. This is a pseudo‐solid‐phase synthesis; only a fraction of the α‐methyl glucoside and the product are soluble in acetone. Molecular sieves were used to remove traces of water in the reaction medium and to increase enzyme stability by removing the acetaldehyde by‐product. A general method was also developed to purify and recover the monoacrylate product from unreacted sugar and undesired diester by a simple crystallization and precipitation process. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 15–22, 2002.