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Removal of the fermentation by‐product succinyl L ‐tyrosine from the β‐lactamase inhibitor clavulanic acid using a molecularly imprinted polymer
Author(s) -
Yu Yihua,
Ye Lei,
de Biasi Vern,
Mosbach Klaus
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10271
Subject(s) - clavulanic acid , chemistry , molecularly imprinted polymer , fermentation , methacrylic acid , tyrosine , molecular imprinting , chromatography , penicillin , natural product , biochemistry , chloride , polymer , residue (chemistry) , monomer , nuclear chemistry , antibiotics , organic chemistry , catalysis , selectivity , amoxicillin
Clavulanic acid is a β–lactamase inhibitor used in therapeutic combinations with the penicillin‐type antibiotics. During the fermentation leading to clavulanic acid, a succinyl L ‐tyrosine by‐product is unavoidably formed. Occasionally, the amount of this by‐product is found to be as high as 2% of the product even after standard purification operations. To further remove this impurity, we prepared a highly specific adsorbent for succinyl L ‐tyrosine with the molecular imprinting technique. This was performed by simultaneously using vinylbenzyl trimethylammonium chloride and methacrylic acid as the functional monomers. The imprinted polymer selectively bound succinyl L ‐tyrosine, and could be successfully used to remove this impurity at concentrations of less than 2% in the presence of clavulanic acid. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 23–28, 2002.