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Kinetic analysis of high‐concentration isopropanol biodegradation by a solvent‐tolerant mixed microbial culture
Author(s) -
Bustard Mark T.,
Meeyoo Vissanu,
Wright Phillip C.
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10246
Subject(s) - biodegradation , substrate (aquarium) , solvent , kinetics , chemistry , population , substrate specificity , carbon source , bioreactor , bacterial growth , chromatography , organic solvent , enzyme , chemical engineering , organic chemistry , biochemistry , bacteria , biology , ecology , physics , demography , genetics , quantum mechanics , sociology , engineering
The ability of a previously enriched microbial population to utilize isopropanol (IPA) as the sole carbon source within a minimal salts medium is studied. The advantage of prior enrichment procedures for the improvement of IPA biodegradation performance is demonstrated for an IPA concentration of up to 24 g L −1 . Results showing the interrelationship between temperature and substrate utilization and inhibition levels at temperatures of between 2°C and 45°C are examined. Models of inhibition based on enzyme kinetics are assessed via nonlinear analysis, in order to accurately represent the growth kinetics of this solvent‐tolerant mixed culture. The model that best describes the data is the Levenspiel substrate inhibition model, which can predict the maximum substrate level above which growth is completely limited. This is the first report of IPA treatment of up to 24 g L −1 by an aerobic solvent‐tolerant population. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 708–713, 2002.