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Decreasing the hyphal branching rate of Saccharopolyspora erythraea NRRL 2338 leads to increased resistance to breakage and increased antibiotic production
Author(s) -
Wardell J. N.,
Stocks S. M.,
Thomas C. R.,
Bushell M.E.
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10210
Subject(s) - hypha , breakage , mutant , chemistry , branching (polymer chemistry) , microbiology and biotechnology , in vitro , antibiotics , biology , biochemistry , gene , materials science , organic chemistry , composite material
Mutation and selection for increased resistance to cell‐wall synthesis inhibitors led to alterations in the hyphal branching rate of Saccharopolyspora erythraea NRRL 2338. Mutants with decreased branching frequency exhibited increased hyphal strength (estimated by in vitro micromanipulation). As the hyphal strength was increased, this led to a greater proportion of hyphal particles in liquid culture with a hyphal fragment diameter of greater than 88 μm. This, in turn, coincided with proportionately increased antibiotic production. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 141–146, 2002; DOI 10.1002/bit.10210