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A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen‐printed electrode
Author(s) -
Koblížek Michal,
Malý Jan,
Masojídek Jiří,
Komenda Josef,
Kučera Tomáš,
Giardi Maria T.,
Mattoo Autar K.,
Pilloton Roberto
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10190
Subject(s) - biosensor , simazine , detection limit , atrazine , chemistry , photosystem ii , electrode , electron acceptor , triazine , chromatography , nuclear chemistry , photochemistry , photosynthesis , biochemistry , organic chemistry , pesticide , agronomy , biology
A biosensor for the detection of triazine‐ and phenylurea‐type herbicides was constructed using isolated Photosystem II (PS II) complexes as a biosensing element. PSII isolated from the thermophilic cyanobacterium Synechococcus elongatus was immobilized on the surface of a screen‐printed sensor composed of a graphite working electrode and Ag/AgCl reference electrode deposited on a polymeric substrate. The biosensor was mounted in a flow microcell with illumination. The principle of the detection was based on the fact that herbicides selectively block PSII electron transport activity in a concentration‐dependent manner. Changes of the activity were registered amperometrically as the rate of photoreduction of an artificial electron acceptor. The setup resulted in a reusable herbicide biosensor with a good stability (half‐life of 24 h) and limit of detection of approximately 10 −9 M for diuron, atrazine and simazine. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 110–116, 2002; DOI 10.1002/bit.10190