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Continuous protein recovery from whey using liquid‐solid circulating fluidized bed ion‐exchange extraction
Author(s) -
Lan Qingdao,
Bassi Amarjeet,
Zhu JingXu Jesse,
Margaritis Argyrios
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10171
Subject(s) - fluidized bed , whey protein , chromatography , extraction (chemistry) , effluent , chemistry , ion exchange resin , ion exchange , whey protein isolate , protein purification , fluidized bed combustion , ion , environmental science , environmental engineering , organic chemistry
A liquid‐solid circulating fluidized bed (LSCFB) continuous ion‐exchange extraction system has been investigated for total protein recovery from whey solutions under various operating conditions. The effectiveness of a dynamic seal was evaluated between the riser and the downcomer, and the best conditions for the establishment of this seal were established. Start‐up studies indicated that the system is robust and stable. Under optimal conditions, a productivity of 8.2 g of total protein removed per hour per kilogram of resin was achieved with a protein removal efficiency of 78.4%. However, higher overall protein recovery of up to 90% was also achieved under other conditions, with lower protein concentration in the effluent and a lower overall productivity. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 157–163, 2002; DOI 10.1002/bit.10171