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Screening of various glycosidases for the synthesis of octyl glucoside
Author(s) -
Ducret Amélie,
Trani Michael,
Lortie Robert
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10156
Subject(s) - chemistry , thermophile , hydrolysis , glucoside , alkyl , enzyme , glycoside hydrolase , octyl glucoside , mesophile , organic chemistry , glycoside , condensation , enzymatic hydrolysis , biocatalysis , biochemistry , catalysis , bacteria , biology , reaction mechanism , medicine , physics , alternative medicine , pathology , genetics , thermodynamics
Thirteen glycosidases of microbial origin and almond β‐glycosidase were assayed in octanol/DMF (80:20, v/v), using a combination of hydrolysis, transglycosylation, and condensation reactions, in order to assess their potential for the production of alkyl glucosides. The two mesophile enzymes were highly impaired by the organic media. Three of the 11 thermophile enzymes gave interesting results in the hydrolysis and transglycosylation reactions, but they were highly inhibited by glucose. This made their use in a condensation reaction less interesting than the use of almond β‐glucosidase, which has a lower activity but shows less inhibition by the glucose. © 2002 Government of Canada. Exclusive worldwide publication rights in this article have been transferred to John Wiley & Sons, Inc. Biotechol Bioeng 77: 752–757, 2002; DOI 10.1002/bit.10156