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Overexpression of alcohol dehydrogenase or pyruvate decarboxylase improves growth of hairy roots at reduced oxygen concentrations
Author(s) -
Shiao Tienli,
Ellis Marc H.,
Dolferus Rudy,
Dennis Elizabeth S,
Doran Pauline M
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10147
Subject(s) - pyruvate decarboxylase , alcohol dehydrogenase , pyruvate dehydrogenase complex , oxygen , chemistry , pyruvate dehydrogenase phosphatase , pyruvate decarboxylation , biochemistry , pyruvate dehydrogenase kinase , enzyme , organic chemistry
Overexpression of Arabidopsis thaliana genes for the fermentation enzymes, alcohol dehydrogenase and pyruvate decarboxylase, improved the tolerance of A . thaliana hairy roots to low oxygen conditions. Whereas the specific growth rate of untransformed hairy roots in shake flasks and in a multiple‐tube recirculation bioreactor declined significantly with decreasing oxygen tension down to 25% air saturation, growth of the transformant root lines was maintained at rates similar to those achieved with full aeration. This work demonstrates that altering the expression of selected genes involved in anaerobic metabolism can alleviate the problems of oxygen deficiency in hairy root cultures caused by poor mixing and mass transfer conditions. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 455–461, 2002; DOI 10.1002/bit.10147