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Self‐assembly and two‐dimensional patterning of cell arrays by electrophoretic deposition
Author(s) -
Brisson Vanessa,
Tilton Robert D.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10146
Subject(s) - electrophoretic deposition , electrophoresis , electrode , electric field , monolayer , deposition (geology) , materials science , nanotechnology , diffusion , colloid , chemistry , chemical engineering , chromatography , paleontology , physics , thermodynamics , quantum mechanics , sediment , engineering , biology , coating
Using Saccharomyces cerevisiae as a demonstration system, we present a method to form two‐dimensional, patternable cellular arrays. The method does not require surface chemical templating of the substratum to produce arrays or patterns. By virtue of their colloidal characteristics, S. cerevisiae cells may be induced to form dense, quasi‐ordered two‐dimensional clusters adjacent to an electrode surface by electrophoretic deposition (EPD). Using ac EPD, dense two‐dimensional cell clusters may be formed in minutes from extremely dilute cell suspensions. The arrays may be induced to form geometric patterns by focusing the electric field during deposition. These monolayer arrays are reversible, dissipating by diffusion on removal of the electric field, and are not in adhesive contact with the electrode surface. Brief application of a modest dc current density adheres the arrays tightly to the surface. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 290–295, 2002.