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The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell–baculovirus expression system
Author(s) -
Zhang Fuming,
Saarinen Mark A.,
Itle Laura J.,
Lang Sybil C.,
Murhammer David W.,
Linhardt Robert J.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10131
Subject(s) - recombinant dna , glycosylation , insect , protein expression , baculoviridae , oxygen , biochemistry , chemistry , cell , biology , microbiology and biotechnology , spodoptera , botany , gene , organic chemistry
The effect of dissolved oxygen concentration on human secreted alkaline phosphatase (SEAP) glycosylation by the insect cell–baculovirus expression system was investigated in a well‐controlled bioreactor. Oligomannose‐type N‐linked glycans (i.e., Man2 to Man6 and Man3F) were present in SEAP produced by Spodoptera frusiperda Sf‐9 (Sf‐9) and Trichoplusia ni BTI‐Tn‐5B1‐4 (Tn‐5B1‐4) insect cell lines. The relative amounts of the most highly processed glycans (i.e., Man3F and Man2 in the SEAP from Sf‐9 and Tn‐5B1‐4 cells, respectively) were significantly higher at 50% of air saturation than at either 10% or 190% of air saturation. That is, glycan processing was inhibited at both low and high dissolved oxygen concentrations. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 219–224, 2002.