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Kinetics of baculovirus replication and release using real‐time quantitative polymerase chain reaction
Author(s) -
Rosinski Matthew,
Reid Steven,
Nielsen Lars Keld
Publication year - 2002
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10126
Subject(s) - real time polymerase chain reaction , polymerase chain reaction , replication (statistics) , viral replication , budding , biology , dna replication , baculoviridae , kinetics , polymerase , virus , dna , virology , microbiology and biotechnology , genetics , gene , recombinant dna , physics , quantum mechanics , spodoptera
The study of viral‐based processes is hampered by (a) their complex, transient nature, (b) the instability of products, and (c) the lack of accurate diagnostic assays. Here, we describe the use of real‐time quantitative polymerase chain reaction to characterize baculoviral infection. Baculovirus DNA content doubles every 1.7 h from 6 h post‐infection until replication is halted at the onset of budding. No dynamic equilibrium exists between replication and release, and the kinetics are independent of the cell density at the time of infection. No more than 16% of the intracellular virus copies bud from the cell. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 476–480, 2002; DOI 10.1002/bit.10126