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Bioengineering and characterization of DNA–protein assemblies floating on supported membranes
Author(s) -
Boireau Wilfrid,
Bombard Sophie,
Sari MarieAgnès,
Pompon Denis
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10111
Subject(s) - characterization (materials science) , surface plasmon resonance , dna , biosensor , nanotechnology , membrane , nanostructure , electrophoresis , chemistry , materials science , biophysics , biology , biochemistry , nanoparticle
Abstract A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis ‐platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra‐assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self‐organization and fluidity properties and is of interest as a prototype to design new generations of DNA chip biosensors. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 225–231, 2002.