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Exploitation of a library of α‐galactosidases for the synthesis of building blocks for glycopolymers
Author(s) -
Casali Monica,
Tarantini Letizia,
Riva Sergio,
Hunkova Zdenka,
Weignerova Lenka,
Kren Vladimir
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10101
Subject(s) - aspergillus terreus , chemistry , hydrolysis , aspergillus , enzyme , penicillium , galactosidases , stereochemistry , biochemistry , organic chemistry , biology , food science , beta galactosidase , microbiology and biotechnology , gene expression , gene
After screening a library of fungal α‐galactosidases for the synthesis of functionalized alkyl α‐ D ‐galactopyranosides, four enzymes (isolated from Aspergillus terreus CCM55, Aspergillus commune CCM 2969, Penicillium vinaceum CCM 2384, or Penicillium brasilianum 2155) proved to be suitable for these biotransformations. The effect of different concentrations of alcohol on activity and stability of these enzymes was investigated. After optimization of the reaction conditions, three galactose derivatives (allyl, 2‐nitroethyl and 2‐(2′,2′,2′‐trifluoroacetamido)‐ethyl α‐ D ‐galactopyranoside, 1a, 3a, and 4a, respectively), suitable for subsequent chemical polymerization, were synthesized using either the “reverse hydrolysis” or the “transglycosylation” protocols. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77:105–110, 2002.