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Using ATP bioluminescence technique for monitoring microbial activity in sludge
Author(s) -
Chu C. P.,
Lee D. J.,
Chang BeaVen,
Liao C. S.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10076
Subject(s) - bioluminescence , activated sludge , adenosine triphosphate , chemistry , heterotroph , bacteria , suspended solids , biochemistry , environmental chemistry , chromatography , biophysics , environmental science , sewage treatment , biology , environmental engineering , wastewater , genetics
This study demonstrates, for the first time, that the presence of suspended solids in waste‐activated sludge interferes with adenosine triphosphate (ATP) bioluminescence tests. The sludge subject to acid/alkaline treatment represented the test sample. Without consideration of the effect of solid concentrations, one would erroneously estimate the density levels of heterotrophic bacteria in the sludge using ATP data. A light blockage model was proposed to evaluate the luminescence reading without the interference of suspended solids. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 469–474, 2001.