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Influence of water activity on the enantioselective esterification of (R,S)‐ibuprofen by crosslinked crystals of Candida antarctica lipase B in organic solvent media
Author(s) -
Pepin Pierre,
Lortie Robert
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10051
Subject(s) - candida antarctica , lipase , dodecanol , chemistry , glutaraldehyde , solvent , enantioselective synthesis , ibuprofen , organic chemistry , water activity , chromatography , catalysis , enzyme , water content , medicine , pharmacology , geotechnical engineering , engineering
The lipase B from Candida antarctica was purified from a commercial source and crystalized. The microcrystals were crosslinked using glutaraldehyde. The crosslinked crystals were then used to catalyze the esterification of ( R,S )‐ibuprofen with dodecanol in octane at various water activities. As for the commercial preparation immobilized on acrylic resin, Novozym 435, low water activities foster better enantioselectivity, and the maximum reaction rates are obtained for a W = 0.1. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 559–562, 2001.