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Multiple stable states and hysteresis in continuous, oscillating cultures of budding yeast
Author(s) -
Zamamiri AbdelQader M.,
Birol Gülnur,
Hjortsø Martin A.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10038
Subject(s) - dilution , yeast , steady state (chemistry) , budding yeast , budding , hysteresis , dynamics (music) , operating point , chemistry , chemical physics , biophysics , physics , thermodynamics , biological system , biology , saccharomyces cerevisiae , biochemistry , condensed matter physics , genetics , engineering , acoustics , electrical engineering
The conditions that precede the onset of autonomous oscillations in continuous yeast cultures were studied in three different types of experiments. It was found that the final state of the culture depended on the protocol used to start up the reactor. Batch cultures, switched to continuous operation at different stages of the batch growth curve, all exhibited similar dynamics—ethanol depletion followed by autonomous oscillations. Small perturbations of the distribution of states in the reactor, achieved by addition of externally grown cells, were able to quench the oscillatory dynamics. Reaching the desired operating point by slow dilution rate changes gave rise to different final states, two oscillatory states and one steady state, depending on the rate of change in dilution rate. The multiplicity of stable states at a single operating point is not explained by any current distributed model and points toward a segregated mechanism of these oscillations. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 305–312, 2001.