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Effects and mechanisms of H 2 O 2 on production of dicarboxylic acid
Author(s) -
Jiao Peng,
Huang Yingming,
Li Shuliang,
Hua Yutao,
Cao Zhu'an
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10027
Subject(s) - fermentation , bioreactor , hydrogen peroxide , chemistry , aeration , oxygen , candida tropicalis , peroxide , biochemistry , yeast , organic chemistry
Abstract The system of producing long chain dicarboxylic acid (DCA) by Candida tropicalis is an aerobic and viscous fermentation system. A method to overcome the gas‐liquid transport resistance and to increase oxygen supply is by adding hydrogen peroxide (H 2 O 2 ) to the fermentation system. Here we report that the H 2 O 2 not only can enhance the oxygen supply but also change the metabolism by inducing cytochrome P450, the key enzyme of a, o‐oxidation. When C. tropicalis was cultivated in a 3‐L bioreactor using the combination of aeration and H 2 O 2 feeding, DCA production rates increased by about 10% after a short period of decrease at the beginning. Furthermore, the experiments showed that the maximum activities of P450 could be induced at 2 mM H 2 O 2 , and the inducible mechanisms are also discussed. Moreover, we suggest that alkane might be oxidized through the “peroxide shunt pathway” when H 2 O 2 is present. By adding H 2 O 2 , the DCA yield in a 22‐L bioreactor could increase by 25.3% and reach 153.9 g/L. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 456–462, 2001.