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Decolorization of reactive dyes by the white rot fungus Trametes versicolor in sequencing batch reactors
Author(s) -
Borchert Maren,
Libra Judy A.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10026
Subject(s) - trametes versicolor , mycelium , erlenmeyer flask , chemistry , population , laboratory flask , food science , chromatography , nuclear chemistry , microbiology and biotechnology , botany , biochemistry , laccase , biology , organic chemistry , enzyme , demography , sociology
The white rot fungus Trametes versicolor was shown to be capable of decolorizing three reactive dyes in a sequencing batch process, using glucose as the carbon and energy source over an extended period without supplementation of new mycelium. Decolorization activity was related to the expression of extracellular peroxidases and could be continuously reactivated by sheering the suspended pellets. Pure culture experiments were carried out simultaneously in agitated Erlenmeyer flasks and in completely stirred tank reactors with two azo dyes, C.I. Reactive Black 5 and C.I. Reactive Red 198 as well as the anthraquinone dye C.I. Reactive Blue 19 (Brilliant Blue R). Results show high and stable degrees of decolorization of 91%–99% in both systems, which could be repeated without decrease in activity over time. Under nonsterile conditions only five cycles of decolorization could be achieved. An increasing bacterial population suppressed fungal growth and the formation of peroxidases. © John Wiley & Sons, Inc. Biotechnol Bioeng 75: 313–321, 2001.