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Water‐based nanoparticulate polymeric system for protein delivery
Author(s) -
Prokop Ales,
Holland Celia A.,
Kozlov Evgenii,
Moore Billy,
Tanner Robert D.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10025
Subject(s) - nanoparticle , nanotechnology , drug delivery , continuous flow , supramolecular chemistry , polymer , continuous production , gene delivery , materials science , chemistry , molecule , biochemical engineering , organic chemistry , engineering , composite material , biochemistry , genetic enhancement , gene
This article features a new production technology for nanoparticles comprised of multicomponent polymeric complexes that are candidates for delivery vehicles of biological molecules such as proteins and drugs. Biocompatible and mostly natural polymers are fabricated into thermodynamically stable nanoparticles insoluble in water and buffered media, in the absence of organic solvents, using two types of processing: batch and continuous. Careful choice of construction materials and the superposition of several interacting principles during their production allow for the customization of the physicochemical properties of the structures. Detailed experiments in batch and continuous systems allowed time‐dependent stoichiometric characterization of the production process and an understanding of fundamental assembly principles of such supramolecular structures. Continuous‐flow production is shown to provide more consistent data in terms of product quality and consistency, with further possibility of process development and commercialization. The development of nanoparticles using the described methodology is expected to lead to a flexible nanoparticle drug delivery system for medical applications, which has particular bearing to the slow release of drugs, antigens (for vaccine design), and genes (for gene therapy). Several chemistries of particles are presented. © John Wiley & Sons, Inc. Biotechnol Bioeng 75: 228–232, 2001.