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Glucose‐based optimization of CHO‐cell perfusion cultures
Author(s) -
Dowd Jason E.,
Kwok K. Ezra,
Piret James M.
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10013
Subject(s) - perfusion , set point , chemistry , glucose uptake , cell culture , biomedical engineering , chromatography , biology , medicine , insulin , endocrinology , control engineering , engineering , genetics
Perfusion cultures of CHO cells producing t‐PA were performed using acoustic filter cell retention. A robust off‐line glucose analysis and predictive control protocol was developed to maintain the process within approximately 0.5 m M of the glucose set point, without the need for a more fallible on‐line sensor. Glucose usage (the difference between the inlet and reactor glucose concentrations) provided an easily measured indicator of overall medium utilization for mapping acceptable ranges of operation, including the edge of failure. Earlier onset of perfusion with a ramping glucose set point (1.5 m M /d) resulted in improved growth and consistency during the perfusion culture start‐up. At steady state, the t‐PA concentration variability increased gradually with increasing glucose usage up to approximately 22 m M, then up to 24 m M the variability increased threefold. Peak t‐PA concentrations of over 90 mg/L were obtained by controlling at a glucose usage of approximately 24 m M, but these t‐PA levels were not sustainable for more than 3 days. A consistent t‐PA concentration of 40 mg/L was obtained at a glucose usage of 21.5 m M. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 75: 252–256, 2001.