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Cell cycle‐dependent protein secretion by Saccharomyces cerevisiae
Author(s) -
Frykman Scott,
Srienc Friedrich
Publication year - 2001
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.10003
Subject(s) - secretion , cell cycle , saccharomyces cerevisiae , microbiology and biotechnology , biology , cell division , cell , heterologous , cell culture , population , biochemistry , yeast , genetics , gene , demography , sociology
Synchronized Saccharomyces cerevisiae cell populations were used to examine secretion rates of a heterologous protein as a function of cell cycle position. The synchronization procedure had a profound effect on the type and quality of data obtained. When cell synchrony was induced by cell cycle‐arresting drugs, a significant physiological perturbation of cells was observed that obscured representative secretion data. In contrast, synchronization with centrifugal elutriation resulted in synchronized first‐generation daughter cells with undetectable perturbation of the physiological state. The synchronized cells did not secrete significant amounts of protein until they reached cell division, suggesting that the secretion process in these cells is strongly cell cycle dependent. However, the maximum secretion rate of the synchronized culture (7–14 molecules/cell/second) was significantly lower than that of an asynchronous culture (29–51 molecules/cell/second). This result indicates that young daughter cells isolated in the synchronization process exhibit different protein secretion behavior than older mother cells that are absent in the synchronized cell population but present in the asynchronous culture. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 76: 259–268, 2001.