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One‐bead–one‐structure combinatorial libraries
Author(s) -
Lebl Michal,
Krchňák Viktor,
Sepetov Nikolai F.,
Seligmann Bruce,
Štrop Peter,
Felder Stephen,
Lam Kit S.
Publication year - 1995
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360370303
Subject(s) - edman degradation , chemistry , combinatorial chemistry , computational biology , scaffold , bead , peptide library , nanotechnology , computer science , biochemistry , peptide sequence , materials science , programming language , biology , gene , composite material
Combinatorial libraries employing the one‐bead–one‐compound technique are reviewed. Two distinguishing features characterize this technique. First, each compound is identified with a unique solid support, enabling facile segregation of active compounds. Second, the identity of a compound on a positively reacting bead is elucidated only after its biological relevance is established. Direct methods of structure identification (Edman degradation and mass spectroscopy) as well as indirect “coding” methods facilitating the synthesis and screening of nonpeptide libraries are discussed. Nonpeptide and “scaffold” libraries, together with a new approach for the discovery of a pentide binding motif using a “library of libraries,” are also discussed. In addition, the ability to use combinatorial libraries to optimize initially discovered leads is illustrated with examples using peptide libraries. © 1994 John Wiley & Sons, Inc.