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The use of spectral decomposition via the convex constraint algorithm in interpreting the CD‐observed unfolding transitions of C coils
Author(s) -
Holtzer Marilyn Emerson,
Holtzer Alfred
Publication year - 1995
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360360310
Subject(s) - tropomyosin , chemistry , spectral line , conformational isomerism , extant taxon , crystallography , regular polygon , algorithm , molecule , physics , geometry , mathematics , actin , organic chemistry , biology , biochemistry , astronomy , evolutionary biology
Decomposition of CD spectra for the unfolding of both coiled‐coil and single‐helical molecules is carried out via the convex constraint algorithm (CCA) [A. Perczel, M. Hollósi, G. Tusnády, and G. D. Fasman (1991) Protein Engineering, Vol. 4, pp. 669–679]. Examined are (1) our thermal unfolding data for rabbit αα‐tropomyosin and chicken gizzard γγ‐ tropomyosin coiled coils, and for a35‐residue, tropomyosin‐model peptide that forms single helices, not coiled coils; (2) extent pH‐induced unfolding data for 50‐ and 400‐residue poly‐ L ‐glutamic acid. Each set of spectra shows a sharp isodichroic point near 203 nm. We find here that the CCA is of sharply limited use for analyzing such data. The component spectra obtained for a given substance not only depend on the particular experimental spectra included and on the chosen number of component spectra, but all pass through the experimental isodichroic point. The latter is physically unlikely for more than three component spectra, and physically impossible for conformers, such as β structures, having known isodichroic points elsewhere. Our conclusions are in contrast to those of an extant decomposition via CCA of thermal spectra for rabbit αα‐tropomyosin [N. J. Greenfield and S. E. Hitchcock‐DeGregori(1993) Protein Science, Vol. 2, pp. 1263‐1273] that postulates the existence of five conformers, including β structures, in the unfolding. Moreover, an extant diagnostic based on the θ 222 /θ 208 ratio and allegedly distinguishing between spectra for coiled coil and for single α‐helix is shown here to be unreliable. © 1995 John Wiley & Sons, Inc.