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Comparison of base inclination in ribo‐GC and deoxybribo‐GC polymers, and synthesis of poly(rGrC)‐poly(rGrC)
Author(s) -
Jin Xiaokui,
Johnson W. Curtis
Publication year - 1995
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360360306
Subject(s) - inclination angle , chemistry , polymer , base (topology) , circular dichroism , buffer (optical fiber) , helix (gastropod) , crystallography , linear dichroism , stereochemistry , geometry , organic chemistry , mathematical analysis , telecommunications , ecology , mathematics , snail , computer science , biology
The inclination angle between the base normal and the helix axis, and the axes around which the bases incline, are measured for ribo‐GC polymers in buffer by using flow linear dichroism (LD), and compared to measurements for deoxyribo‐GC polymers in buffer and under dehydrating conditions. A new method is designed to synthesize poly(rGrC) ‐poly(rGrC), which is not available commercially, in large quantities. The LD of this RNA reveals inclination angles that are similar to the B‐form DNA in buffer, although the axes are different. The CD of poly(dGdC)‐poly(dGdC) under the dehydrating conditions is similar to poly(rGrC)‐poly(rGrC), indicating it is in the A form, and the LD gives larger inclination angles than either the B form or the corresponding RNA. Poly(dG)‐poly(dC) is in the A form in buffer. Comparison among poly(rG)‐poly(rC) in buffer, and poly (dG)‐poly(dC) in buffer and under dehydrating conditions, reveals similar inclination angles and axes, although the LD shows that the DNA has the largest inclination angles. Except for poly(rGrC)‐poly(rGrC), which has a unique reduced dichroism, all the axes for G are similar, as are the axes for C. © 1995 John Wiley & Sons, Inc.