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Comparison of base inclination in ribo‐AU and deoxyribo‐AT polymers
Author(s) -
Jin Xiaokui,
Johnson W. Curtis
Publication year - 1995
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360360305
Subject(s) - polymer , chemistry , base (topology) , solvent , helix (gastropod) , circular dichroism , inclination angle , polymer chemistry , crystallography , organic chemistry , geometry , mathematical analysis , ecology , mathematics , snail , biology
The inclination angle between the base normal and the helix axis is measured for ribo‐AU polymers by using flow linear dichroism (LD), and compared to measurements for deoxyribo‐AT polymers under dehydrating conditions. The CD of the DNA polymers under the dehydrating conditions is not the same as the corresponding RNA polymers, which are presumed to be in the A form. However, the LD indicates that poly(dAdT)‐poly(dAdT) can assume the A form in 80% 2,2,2‐trifluoroethanol, although poly(dA)‐poly(dT) retains B form structure in this dehydrating solvent. The inclination angles are similar for B form poly(dAdT)‐poly(dAdT) and poly(dA)‐poly(dT), and these parameters are also similar for A form poly(rArU) ‐poly(rArU) and poly(rA) ‐poly(rU). All the inclination axes are similar. © 1995 John Wiley & Sons, Inc.