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Duplex to quadruplex equilibrium of the self‐complementary oligonucleotide d(GGGGCCCC)
Author(s) -
Deng Hong,
Braunlin William H.
Publication year - 1995
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360350613
Subject(s) - chemistry , duplex (building) , oligonucleotide , hydrogen bond , g quadruplex , titration , crystallography , dna , biochemistry , organic chemistry , molecule
The structure of the deoxyaligonucleotide d(GGGGCCCC) has been monitored by 1 H and 31 P NMR, and by gel electrophoresis. In low‐salt solution, this oligonucleotide forms a stable duplex structure. Upon titration with KCl, an equilibrium is established between duplex and quadruplex forms. The quadruplex form is the dominant one at physiological KCl concentrations, despite the fact that fewer hydrogen bonds are formed per strand in the quadruplex than in the duplex. © 1995 John Wiley & Sons, Inc.