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Gel electrophoresis measurement of counterion condensation on DNA
Author(s) -
Ma Chenglie,
Bloomfield Victor A.
Publication year - 1995
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360350209
Subject(s) - counterion , chemistry , counterion condensation , electrophoresis , agarose gel electrophoresis , valence (chemistry) , agarose , tris , gel electrophoresis , dna , chromatography , ion , organic chemistry , biochemistry
We used agarose gel electrophoresis to measure the effective charge neutralization of DNA by counterions of different structure and valence, including Na + , Mg 2+ , Co(NH 3 ) 6 3+ , and sperinidine 3+ , which competed for binding with an excess of Tris acetate buffer. Linear DNA molecules ranged in size from 1 to 5 kilobases, and supercoiled plasmid pUC18 was also measured. In all cases, the results were in good agreement with theoretical predict ions from counterion condensation theory for two‐counterion mixtures. © 1995 John Wiley & Sons, Inc.