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Conformational studies of an undecapeptide reproducing the consensus sequence around the cleavage site of the RXVRG endoprotease from Xenopus laevis skin
Author(s) -
Baron Daniel,
Leseney AnneMarie,
Chalaoux FrancoisRegis,
Riand Jacques
Publication year - 1994
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360341013
Subject(s) - chemistry , intramolecular force , conformational isomerism , stereochemistry , nuclear magnetic resonance spectroscopy , hydrogen bond , cleavage (geology) , two dimensional nuclear magnetic resonance spectroscopy , sequence (biology) , nuclear overhauser effect , crystallography , molecule , biochemistry , geotechnical engineering , organic chemistry , fracture (geology) , engineering
Two synthetic fragments, corresponding to the 4–9 and 4–14 sequences of a tetradecapeptide used as a model to test the RXVRG‐endoprotease activity from Xenopus laevis skin, have been studied by two‐dimensional nmr spectroscopies, correlated spectroscopy, and nuclear Overhauser effect (NOE) spectroscopy. Both peptides wore the 5–9 consensus sequence found in several hormonal precursors. The nmr data for the 4–9 hexapeptide did not indicate any particular organization, either in water or in dimethylsulfoxide (DMSO), whereas, the 4–14 undecapeptide, a substrate for the RXVRG endoprotease, showed, in DMSO solution, significant trends of structural organization involving the amino acids pertaining to the consensus domain. From variations of integrated NOE peaks with temperature, the appearent interproton correlation times τ c were estimated and the maxima observed with Va17, the central residue in the consensus sequence. A defined tertiary structure in that domain was also supported by medium‐and long‐range NOEs between As6 and Arg8, Glu4 and Gly9, and by the likely involvement of Arg8 and Gly9 NHs in intramolecular hydrogen bonds. Most of these observations could be rationalized by an equilibrium between a 5–3 β‐turn and a 9 → 4 H‐bonded loop. The predominance of one rotamer for the Cα‐Cβ bond was established in four residues. Finally, the average ϕ and ψ angles were derived from two models taking, or not, into account variations in the correlation times along the sequence. This allowed us to discuss the artifacts generated by using an average correlation time through the whole molecule. © 1994 John Wiley & Sons, Inc.