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Effect of cationic drugs on suprahelical organization of DNA
Author(s) -
Krishna K. Gopala,
Kumar T. K. Suresh,
Pandit M. W.
Publication year - 1993
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360330911
Subject(s) - chemistry , cationic polymerization , dna , computational biology , pharmacology , combinatorial chemistry , biophysics , biochemistry , organic chemistry , medicine , biology
Interaction of a minor groove‐binding drug Hoechst‐33258, and an intercalating drug, proflavin, with the PSI (+) form of DNA, was studied using CD spectroscopy. Both drugs are shown to relax the suprahelical organization of DNA, leading to the formation of a B‐like structure, above a certain drug to phosphate ratio. However, unlike proflavin, Hoechst‐33258 brings about further structural changes after formation of the B‐like structure whereas proflavin does not. A reversal of the CD signal in the 300–450‐nm spectral region is also observed with Hoechst‐33258, indicating a change in the handedness of the suprahelical organization of DNA. To the best of our knowledge, drug‐mediated changes as presented in this paper have not been reported so far. © 1993 John Wiley & Sons, Inc.