The preparation and characterization of a twin‐tailed, lipid‐linked polysialic acid

Reactions between water‐insoluble and water‐soluble components are often slow, giving low yields. In order to prepare glycolipids composed of polysialic acid and N‐(4‐(p‐maleimidylphenyl)butyryl)dipalmitoyl‐ L ‐α‐phosphatidylethanol amine triethylammonium salt, we used liposomes to enhance the interfacial reaction between the reagents. Thus, thiolreactive liposomes were reacted with polysialic acid cysteamine. A 70% yield was achieved with this addition reaction, and the product was isolated by dialysis and centrifugation © 1993 John Wiley & Sons, Inc.