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The use of UV‐visible spectroscopy for the determination of hydrophobic interactions between neuropeptides and membrane model systems
Author(s) -
Young John K.,
Graham William H.,
Beard Debbie J.,
Hicks Rickey P.
Publication year - 1992
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360320815
Subject(s) - chemistry , bathochromic shift , sodium dodecyl sulfate , micelle , aqueous solution , lysophosphatidylcholine , cationic polymerization , membrane , bradykinin , ultraviolet visible spectroscopy , photochemistry , pulmonary surfactant , spectroscopy , organic chemistry , phospholipid , phosphatidylcholine , biochemistry , physics , receptor , quantum mechanics , fluorescence
Ultraviolet‐visible spectroscopy has been used as a rapid method to evaluate the hydrophobia interactions between a series of cationic and zwitterionic neuropeptides and dipeptides with the hydrophobia core of two membrane model systems; sodium dodecyl sulfate and lysophosphatidylcholine micelles. If a hydrophobia interaction occurs, a 1−nm bathochromic shift is observed in the uv‐visible spectrum of the aromatic side chains when going from aqueous solution to a micellar solution. The aromatic residues of substance P, bradykinin, and Des‐Arg 9 bradykinin all exhibited the 1−nm bathochromic shift in the presence of sodium dodecyl sulfate while those of Met‐enkephalin did not. The opposite effects were observed in the presence of lysophosphatidylcholine micelles. © 1992 John Wiley & Sons, Inc.