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Synthesis of new hydrophilic microspheres: Optimized carriers for microparticle‐enhanced nephelometric immunoassays
Author(s) -
Marchand J.,
Varcin P.,
Riochet D.,
Montagne P.,
Cuilliere M. L,
Duheille J.,
Pau B.
Publication year - 1992
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360320808
Subject(s) - chemistry , microsphere , microparticle , hapten , covalent bond , immunoassay , conjugate , antiserum , chromatography , antigen , antibody , chemical engineering , organic chemistry , immunology , mathematics , mathematical analysis , engineering , biology
To optimize antigen–antibody reactions, we have synthesized chemically well‐defined hy‐drophilic microspheres. Proteins or haptens were covalently linked to these carriers. When the microsphere conjugates were agglutinated by the corresponding antiserum, the size of the complex artificially increased during the immunological reaction. After optimizing var‐ious parameters such as the hydrophilic character, repulsion charges, and amount of antigen coupled to the microspheres, we developed a rapid and sensitive immunoassay based on laser light scattering by the complexes.