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Spectroscopic study of the interaction between poly‐(9‐vinyladenine) and single or multistrand RNA
Author(s) -
Yashima Eiji,
Tajima Teruyo,
Miyauchi Noriyuki,
Akashi Mitsuru
Publication year - 1992
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360320709
Subject(s) - chemistry , hydrogen bond , crystallography , rna , proton nmr , stereochemistry , molecule , biochemistry , organic chemistry , gene
The interaction between poly(9‐vinyladenine) (PVAd) and poly[r (U)] was investigated by means of uv, CD, 1 H‐, and 31 P‐nmr spectroscopies. The interaction was dependent on the molecular weight of PVAd determined by uv and CD spectroscopies. Based on imino proton nmr, it was clearly found that PVAd formed the complex with poly [r(U)] by complementary hydrogen bonding. The interaction of PVAd with double‐ and triple‐stranded helices of RNA was also investigated by uv melting behavior and 31 P‐nmr spectroscopy. The results suggested that PVAd could not interact with the double‐stranded poly [r (A)] · poly[r(U)] but did with the triple‐stranded RNA.