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Partial molar volumes of the amino acid side‐chains of proteins in aqueous solution: Some comments on their estimation using partial molar volumes of amino acids and small peptides
Author(s) -
Hedwig Gavin R.
Publication year - 1992
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360320509
Subject(s) - chemistry , molar , side chain , partial molar property , amino acid , aqueous solution , molar concentration , molar ratio , chromatography , organic chemistry , biochemistry , catalysis , polymer , medicine , dentistry
The partial molar volumes of some amino acid side‐chains were determined in two recent studies [Makhatadzes, G. I., et al. (1990) Biopolymers 30 , 1001, and Reading, J. F. & Hedwig, G. R. (1990) J. Chem. Soc. Faraday Trans. 86 , 3117] using partial molar volume data, V 2 0 , in aqueous solution at 25°C for some peptides of sequence Gly‐X‐Gly, where X is an amino acid. These side‐chain partial molar volumes are critically compared with those obtained using V 2 0data for amino acids. It is concluded that side‐chain partial molar volumes calculated using V 2 0data for the tripeptides are better estimates of side‐chain partial molar volumes in proteins than are those determined using V 2 0data for amino acids.