Premium
The interactions of neuropeptides with membrane model systems: A case study
Author(s) -
Hicks Rickey P.,
Beard Debbie J.,
Young John K.
Publication year - 1992
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360320111
Subject(s) - chemistry , membrane , micelle , lysophosphatidylcholine , biophysics , hydrophobic effect , nuclear magnetic resonance spectroscopy , stereochemistry , biochemistry , organic chemistry , phospholipid , aqueous solution , phosphatidylcholine , biology
The interactions between the positively charged neuropeptides substance P (SP), bradykinin (BK), and zwitterionic Met‐enkephalin (ME) neuropeptides, and negatively charged SDS and zwitterionic lysophosphatidylcholine (LPC) membrane model systems, have been investigated using one‐ and two–dimensional nmr experiments. Proton longitudinal relaxation studies were used to characterize these interactions as intrinsic or extrinsic. An extrinsic interaction are similar to those observed for extrinsic membrane proteins. An intrinsic interaction are similar to those observed for intrinsic membrane proteins, and would require that the hydrophobic residues penetrate or insert into the hydrophobic core of the membrane. The interactions between both SP and BK and SDS, based on nmr results, may be characterized as intrinsic, and the interaction between ME and SDS may be characterized as extrinsic. Two–dimensional nuclear Overhauser enhancement spectroscopy experiments proved the insertion of the phenylalanine residues on both SP and BK into the hydrophobic core of SDS micelles. The interaction between SP and BK with LPC based on nmr results are characterized as extrinsic, with the interaction between ME and SDS characterized as weakly intrinsic.