Premium
Effects of Na + on the persistence length and excluded volume of T7 bacteriophage DNA
Author(s) -
Sobel E. S.,
Harpst J. A.
Publication year - 1991
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360311311
Subject(s) - chemistry , persistence length , counterion , bacteriophage , volume (thermodynamics) , radius , counterion condensation , virial coefficient , rayleigh scattering , persistence (discontinuity) , excluded volume , thermodynamics , physics , optics , molecule , ion , polymer , computer security , organic chemistry , escherichia coli , computer science , gene , geotechnical engineering , engineering , biochemistry
Total intensity, Rayleigh light scattering has been used to measure the rms radius, second virial coefficient, persistence length, and excluded volume of homogeneous T7 bacteriophage DNA as a function of Na + concentration (0.005 to 3.0 M ). All parameters decrease sharply as [Na + ] increases, and tend to level off at high Na + . The variation of persistence length with [Na + ] is consistent with predictions from counterion condensation theory.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom