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Effects of Na + on the persistence length and excluded volume of T7 bacteriophage DNA
Author(s) -
Sobel E. S.,
Harpst J. A.
Publication year - 1991
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360311311
Subject(s) - chemistry , persistence length , counterion , bacteriophage , volume (thermodynamics) , radius , counterion condensation , virial coefficient , rayleigh scattering , persistence (discontinuity) , excluded volume , thermodynamics , physics , optics , molecule , ion , polymer , computer security , organic chemistry , escherichia coli , computer science , gene , geotechnical engineering , engineering , biochemistry
Total intensity, Rayleigh light scattering has been used to measure the rms radius, second virial coefficient, persistence length, and excluded volume of homogeneous T7 bacteriophage DNA as a function of Na + concentration (0.005 to 3.0 M ). All parameters decrease sharply as [Na + ] increases, and tend to level off at high Na + . The variation of persistence length with [Na + ] is consistent with predictions from counterion condensation theory.