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Photodegradation of hyaluronic acid: EPR and size exclusion chromatography study
Author(s) -
Lapc̆ík L'.,
Chabrec̆ek P.,
Stas̆ko A.
Publication year - 1991
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360311209
Subject(s) - chemistry , electron paramagnetic resonance , radical , photodegradation , spin trapping , adduct , macromolecule , irradiation , photochemistry , aqueous solution , hyaluronic acid , radiation chemistry , size exclusion chromatography , nuclear magnetic resonance , organic chemistry , catalysis , chemical reaction , photocatalysis , biochemistry , physics , biology , nuclear physics , genetics , enzyme
Photochemically induced radical reactions involving the lateral sequences and the end macromolecular chain groups of hyaluronic acid in aqueous solutions at 293K were studied by EPR spin trapping technique with DMPO (5,5‐dimethylpyrroline‐1‐oxide). In the first 1‐10 minutes of irradiation EPR indicates spin adducts of two carbon centered radicals with the splitting constants of a N = 1.60 mT, a H = 2.51 mT and a N = 1.56 mT, a H = 2.28 mT. After longer irradiation time (over 10 minutes) dominate two further DMPO adducts of radicals centered on hetero–atoms with splitting constants of a N = 1.44 mT, an = 1.60 mT and of a N = 1‐49 mT, a H = 1.49 mT. Simultaneously, molecular weight followed by SEC decreases, suggesting that UV irradiation leads to the breaking of interglycosidic bonds of hyaluronic acid main macromolecular chain.