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Determining molecular weight distributions of antigen–antibody complex by quasi‐elastic light scattering
Author(s) -
Murphy Regina M.,
Yarmush Martin L.,
Colton Clark K.
Publication year - 1991
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360311107
Subject(s) - chemistry , electron microscope , diffusion , work (physics) , molecular dynamics , light scattering , scattering , thermodynamics , crystallography , computational chemistry , physics , optics
Abstract Physiological properties of soluble antigen–antibody (Ag‐Ab) complexes depend in part on the size of the complexes. In previous work, the size distribution and structure of model Ag‐Ab complexes were determined by electron microscopy. In this study, we used constrained regularization analysis of quasi‐elastic light scattering data to estimate molecular weight distributions of model Ag‐Ab complexes. A conformational model was necessary to determine appropriate correlations between molecular weight and diffusion coefficient, and to estimate particle structure factors. Porod–Kratky theory proved to be an adequate conformational model for these purposes. The molecular weight distributions determined by constrained regularization compared favorably with distributions obtained either by electron microscopy or by thermodynamic modeling.