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Experimental and theoretical studies of DNA separations by capillary electrophoresis in entangled polymer solutions
Author(s) -
Grossman Paul D.,
Soane David S.
Publication year - 1991
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360311010
Subject(s) - electrophoresis , chemistry , capillary electrophoresis , hydroxyethyl cellulose , polymer , capillary action , agarose , agarose gel electrophoresis , rheology , quantum entanglement , dna , chromatography , cellulose , thermodynamics , organic chemistry , physics , biochemistry , quantum mechanics , quantum
Studies of electrophoretic separations of DNA restriction fragments by capillary electrophoresis in solutions of (hydroxyethyl) cellulose (HEC) were performed. Rheological studies were used to confirm that the entanglement threshold (Φ*) for the HEC solutions used is approximately 0.004 g/mL, in good agreement with theoretical predictions. A mesh size an order of magnitude smaller than that found in agarose gels (on a per weight basis) was calculated using polymer‐entanglement theory and was confirmed by electrophoretic measurements. Electrophoretic migration was shown to follow the Ogston regime under most conditions. An approach for obtaining smaller mesh sizes is presented.