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An antigenic determinant common to human chorionic somatomammotropin and human growth hormone revealed by limited proteolysis of immune complexes
Author(s) -
Santucci Annalisa,
Rustici Mauro,
Bracci Luisa,
Neri Paolo
Publication year - 1991
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360310903
Subject(s) - epitope , proteolysis , monoclonal antibody , antigen , chemistry , antibody , immune system , peptide sequence , biochemistry , enzyme , biology , immunology , gene
An epitope of human chorionic somatomammotropin for one of the monoclonal antibodies raised against the whole antigen has been identified. We compared the release of peptides from limited proteolysis of the antigen in the presence and absence of the related antibody. Using enzymes of different specificity, we could determine the amino acid sequence that can be considered at least inclusive of the epitope. The monoclonal antibody selected is 100% cross‐reactive with human growth hormone, so the antigenic determinant identified is shared by the two protein hormones.