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The substructure of agarose gels as prepared for electrophoresis
Author(s) -
Whytock Susan,
Finch John
Publication year - 1991
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360310902
Subject(s) - agarose , substructure , chemistry , electron microscope , electrophoresis , agarose gel electrophoresis , biophysics , crystallography , chromatography , biochemistry , dna , optics , physics , structural engineering , engineering , biology
Gelled agarose was observed by freeze‐fracture and electron microscopy. Aggregated double helices of agarose form a fibrous network that showed conformational variations over a range of agarose concentrations (0.3–2%). Diameters of fibers tend to increase with rising concentrations and the appearance of protuberances or cul‐de‐sacs in the network increases with falling concentration.