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The molecular dimensions of G‐actin in solution as studied by dynamic light scattering
Author(s) -
Patkowski A.,
Eimer W.,
Seils J.,
Schneider G.,
Jockusch B. M.,
Dorfmüller Th.
Publication year - 1990
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360301312
Subject(s) - rotational diffusion , chemistry , light scattering , dynamic light scattering , anisotropy , scattering , actin , ellipsoid , diffusion , molecule , molecular physics , crystallography , optics , classical mechanics , chemical physics , thermodynamics , physics , quantum mechanics , organic chemistry , biochemistry , astronomy , nanoparticle
The rotational and the translational diffusion coefficients of G‐actin in solution have been obtained by polarized and depolarized dynamic light scattering, respectively. Modeling the shape of the molecule by a biaxial ellipsoid of revolution, the hydrodynamic dimensions were calculated from the Perrin equations. In completion to previous hydrodynamic measurements, a combination of both light scattering experiments provides direct information about the anisotropic form of G‐actin in solution. The result indicates that the form of G‐actin in solution is not significantly different from the overall structure in the solid state as obtained by x‐ray crystallography.