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Synthesis and 1 H‐nmr studies of α‐deuterated analogues of des ‐Trp 1 , Nle 12 ‐human minigastrin
Author(s) -
Amonraksa Kitti,
Simonetti Mario,
Da RinFioretto Silvio,
Mammi Stefano,
Peggion Evaristo
Publication year - 1990
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360300719
Subject(s) - chemistry , deuterium , amide , stereochemistry , peptide , nuclear magnetic resonance spectroscopy , organic chemistry , biochemistry , physics , quantum mechanics
Three analogues of the tridecapeptide amide H‐Leu‐(Glu) 5 ‐Ala‐Tyr‐Gly‐Nle‐Asp‐Phe‐NH 2 were synthetized with α‐deuterated glutamate residues in specific positions in order to assign unambiguously the 1 H nmr spectrum of the parent peptide in water and in water‐trifluoroethanol mixtures. The synthetic route is described and the assignment illustrated. A previous, tentative assignment based solely on indirect evidence [Mammi, S., Mammi, N. J. & Peggioa, E. (1988) Biochemistry 27 , 1374–1379] was partially modified.