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Excluded volume effects on the partition of macromolecules between two liquid phases
Author(s) -
Zimmerman Steven B.,
Trach Stefan O.
Publication year - 1990
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360300706
Subject(s) - macromolecule , chemistry , polyethylene glycol , excluded volume , volume (thermodynamics) , partition coefficient , macromolecular substances , aqueous solution , partition (number theory) , chromatography , thermodynamics , polymer , organic chemistry , biochemistry , physics , mathematics , combinatorics
Partition parameters of several proteins and other macromolecules are measured in an aqueous two‐phase liquid system containing polyethylene glycol and phosphate buffer. Distribution of macromolecules is a function of the relative volume excluded to the macromolecules in the two phases. A simple model with no adjustable parameters yields covolumes of the macromolecules with the polyethylene glycol. Covolumes are used to estimate effective molecular volumes and the magnitudes of excluded volume effects. The same approach is applied to mixtures of macromolecules.