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Determination of solution conformation of DNA backbone: Application of homonuclear ( J , δ) spectroscopy
Author(s) -
Hosur R. V.,
Chary K. V. R.,
Saran Anil,
Govil Girjesh,
Miles H. Todd
Publication year - 1990
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360290608
Subject(s) - homonuclear molecule , chemistry , oligonucleotide , coupling constant , spectroscopy , crystallography , dihedral angle , dna , analytical chemistry (journal) , molecule , physics , hydrogen bond , organic chemistry , biochemistry , particle physics , quantum mechanics
Homonuclear two‐dimensional ( J ,δ) proton spectroscopy has been suggested as a method for the measurement of 1 H‐ 31 P coupling constants in oligonucleotides. The technique has been applied to a dinucleoside monophosphate G2′p5′C and a deoxydecanucleotide d(ACATCGATGT). PCILO energy calculations have been carried out to find minimum energy conformations with respect to the DNA backbone torsion angle ε, and these have been considered for the interpretation of the observed H3′‐ 31 P coupling constants in oligonucleotides.