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The elastic modulus of fibrin clots and fibrinogen gels: The effect of fibronectin and dithiothreitol
Author(s) -
Procyk R.,
King R. G.
Publication year - 1990
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360290311
Subject(s) - dithiothreitol , factor xiiia , chemistry , fibrin , fibrinogen , fibronectin , factor xiii , coagulation , thrombin , biophysics , reagent , polymer chemistry , thrombelastography , biochemistry , immunology , organic chemistry , platelet , enzyme , medicine , extracellular matrix , biology
The elastic modulus ( G ′) of factor XIIIa induced fibrinogen gels was found to be substantially lower than the G ′ of fibrin gels that were formed by clotting fibrinogen with thrombin. The addition of fibronectin and/or the reducing reagent dithiothreitol (DTT) to the factor XIIIa coagulation mixture led to the formation of a weaker gel structure, while the rigidity of thrombin induced clots was not appreciably affected by the inclusion of the DTT but increased somewhat in the presence of fibronectin. The reasons for the differing clot rigidities are discussed in terms of biochemical mechanisms.