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Characterization of the reaction products of adult human hemoglobin and disuccinimidyloxalate
Author(s) -
Marini M. A.,
Christensen S.,
Snell S.,
Jessee R.,
Medina F.,
Zegna A.
Publication year - 1989
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360281211
Subject(s) - chemistry , hemoglobin , glycolaldehyde , amide , covalent bond , cyanogen bromide , molecule , yield (engineering) , amino acid , polyethylene glycol , methemoglobin , glutaraldehyde , peptide bond , peg ratio , polymer chemistry , combinatorial chemistry , organic chemistry , biochemistry , peptide sequence , catalysis , materials science , finance , economics , metallurgy , gene
Abstract A number of chemically modified hemoglobin preparations have been proposed for use as an emergency resuscitation fluid. The purpose for forming these hemoglobin derivatives is to decrease the oxygen binding (i.e., to increase the P 50 ) and to increase the intravascular retention time. These goals have been met with various degrees of success by using the reaction with pyridoxyl 5‐phosphate to raise the P 50 , followed by the addition of glutaraldehyde to increase circulating half‐life by polymerization. 1,2 Other derivatives have been formed with polyethylene glycol, 3,4 bis‐(3,5‐dibromosalicyl) fumarate, 5,6 glycolaldehyde, 7 and 2‐nor‐2‐formylpyridoxal 5‐phosphate, 8,9 as well as with other compounds. All these derivatives introduce a foreign molecule into the hemoglobin, which may not always be desirable. Recently Tharp and Day 10 used cyanogen to form intersubunit amide cross‐links in hemoglobin without the incorporation of cyanogen. This approach is attractive if the appropriate functional properties can be attained. Takeda et al. 11 showed that equimolar concentrations of amino acids and disuccinimidyloxalate could form peptide bonds in high yield. We report the characteristics of the hemoglobin molecule modified by internal covalent amide bonds, which may be a suitable candidate for a resuscitation fluid.