Premium
Bisintercalation of ditercalinium into a d(CpGpApTpCpG) 2 minihelix: A 1 H‐ and 31 P‐NMR study
Author(s) -
Delepierre M.,
Dinh T. Huynd,
Roques B. P.
Publication year - 1989
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360281207
Subject(s) - chemistry , dimer , chemical shift , crystallography , nuclear overhauser effect , nuclear magnetic resonance spectroscopy , aqueous solution , resonance (particle physics) , proton nmr , two dimensional nuclear magnetic resonance spectroscopy , stereochemistry , nmr spectra database , nuclear magnetic resonance , spectral line , organic chemistry , physics , particle physics , astronomy
The structure of the complex formed in aqueous solution between ditercalinium, a bisintercalating drug, and the self‐complementary hexanucleotide d(CpGpApTpCpG) 2 is investigated by 400‐MHz 1 H‐nmr and 162‐MHz 31 P‐nmr. Whatever the drug to thlix ratio, ditercaliniun occurred in the bound form, whereas free and complexed hexanucleotide and are in slow exchange. This allows unambiguous resonance assignm, ent through two‐dimensional chemical exvhange experiments. The strong upfield shifts measured on most aromatic protons on both drug and bases as well as on DNA imino protons are consistent with bisintercalation of the dimer. Nuclear Overhauser effects observed between drug and nucleeotide protons give a defined geometry for complexation, and suggest a DNA conformational change upon drug binding.